Isolation a d Purification of Ascorbate
نویسنده
چکیده
A screening test was undertaken to isolate microorganisms that produced ascorbate exidase. The E".Z,IMqe.a.C`llg'.W.ai,l,:,dit:iaEh"S".2e,,fi,l・g"ti",`&Of,?・,fuig,a,i,2tr;IL",(:・ld:-,l5,2u,"eIIYIS,O,i,a.`e.d.I・i.O.M,a,FO"i,a.MP,Lea examinations of cultural conditions, optintum conditions for enzyme production were found; strain HI-25 was aerobically cultured by a jar fermenter at 250C in a rnedium containing 5% glycerol, 2% defatted Z?g.bXaft",Si2liltk・2,.T,::,O:O.dLZM,.Sb':lgt.Mi::egeO.'k%,p.",lfiZPdOfr`dmO'Pi2e%cuitt[uSSe2ttlZI,:e2bOy'aan":mOinO:n?l'tumKCs:1faPY, fractionation, column c
منابع مشابه
Tiie Journal of Biological Chemistry
A new procedure has been described for the isolation and purification of ascorbate oxidase from green zucchini squash (Cucurbita pepo meduttosa). The new purification method achieves a more than ZOO-fold purification with about a 14% recovery of the total activity present in the crude juice. The purified enzyme preparations have been found to be homogeneous by the criteria of ultracentrifugal a...
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